Gene expression profile at single nuclei level of cerebellar neurons (Purkinje cells) and glia (Bergmann glia, velate astrocytes and oligodendrocytes) in a transenic mouse model of Spinocerebellar Ataxia Type 1 (SCA1)

Cerebellar glia are affected in SCA1. We used single nuclei RNA sequencing (snRNA-seq) to analyze the gene expression changes in glia in response to neuronal dysfunction in SCA1. Overall design: Cerebellar nuclei were isolated from 12 weeks old SCA1 mice and Wild type (WT) sex matched littermate controls and analyzed using snRNAseq.