DNA sequencing of Medicago truncatula from ATAC-seq experiment - roots of germinated seeds at 1mm, 1mm+PEG, 5mm

We analysed chromatin changes in Medicago (A17) between radicles from germinated tolreant to desiccation (R1P) or icompared to intolerant to desiccation (R1 and R5). Roots at 1mm were dried for 72hours and are desiccation sensitive, roots at 1mm plus incubation in PEG 8000 at -1.7Mpa for 72h at 10°C then dried for 72hours are desiccation tolerant and roots at 5mm dried for 72h are desiccation sensitive Overall design: Each replicate consists of at least 30 radicles at each stages