Primer characteristics.
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1Probe sequences were generated for the secE and yccT real-time PCR primers, but no in vitro testing was performed. The probe sequences are provided here for the benefit of researchers wishing to perform multiplex real-time PCR reactions using these primers. All primers have an optimal annealing temperature of 56°C.2Primer efficiency was calculated from the slope and intercept of the trendline produced following amplification of serial dilutions of genomic DNA from the ATCC strains of each species, as described previously [14].3Amplicon size in base pairs.
创建时间：
2015-12-02
