Molecular variation and phylogeography within European Isothecium alopecuroides and Pseudisothecium myosuroides (Bryophyta, Lembophyllaceae)

Earlier studies on Isothecium s.l. suggested potential interchange of genetic material between species that are nowadays known to belong to separate genera. In this study, we analyze the molecular variation in three molecular markers (nuclear gdp; mitochondrial nad5; and plastid trnG) in a wide selection of Isothecium alopecuroides and Pseudisothecium myosuroides specimens to infer whether this exchange of genetic material is frequent. In addition, we explore the phylogeographic structure of these two species in Europe. Our results show repeated genetic interchange for the nuclear and mitochondrial markers in both species, with intergeneric hybridization as the most probable explanation. The genetic lineages found indicate a complex biogeographical history for I. alopecuroides, including both postglacial immigration from different glacial refugia and developing haplotypes potentially adapted to cold climates. As for P. myosuroides, genetic variants could either indicate a postglacial im..., We included 94 Isothecium alopecuroides and 40 Pseudisothecium myosuroides specimens, and 13 additional members of the Lembophyllaceae lineage to which these two species belong: Isotheciastrum subdiversiforme (1), Isothecium algarvicum (2), Pseudisothecium cristatum (1), P. holtii (3), P. interludens (3), P. prolixum (2), and P. stoloniferum (1). Most sequences were newly generated for the present study, and some came from an earlier study. We studied a portion of the nuclear glyceraldehyde 3-phosphate dehydrogenase (gdp), the mitochondrial nad5 G1 intron (nad5), and the plastid trnGUCC G2 intron (trnG).
Total DNA was extracted using the DNeasy® Plant Mini Kit for DNA isolation from plant tissue (Qiagen) according to the manufacturer’s instructions. PCR was performed using Ready-To-GoTM PCR Beads (Amersham Pharmacia Biotech) in a 25 ml reaction volume, also following the manufacturer’s protocol. PCR protocol for nad5: 1x 95º 5 min; 1x TC 94º 40 sec, 60‑54º 40 sec, 72º 1 min 30 sec; 34x ..., , # Data from: Molecular variation and phylogeography within European Isothecium alopecuroides and Pseudisothecium myosuroides (Bryophyta, Lembophyllaceae)

## Description of the data and file structure

The data includes the following four files: Isothecium alopecuroides_gpd.txt; Isothecium alopecuroides_nad5.txt; Isothecium alopecuroides_trnG.txt; Isothecium alopecuroides_TOTAL.txt

The files include sequence alignments in FASTA format

Usage notes: The positions in the files are as follows:

Isothecium alopecuroides_gpd.txt:
base positions: 1-510
indel coding positions: 512-515

Isothecium alopecuroides_nad5.txt:
base positions: 1-954
indel coding positions: 956-963

Isothecium alopecuroides_trnG.txt:
base positions: 1-557
indel coding positions: 559-567

Isothecium alopecuroides_TOTAL.txt [only samples with all three markers are included in this alignment]:
base positions: 1-510 (gpd); 517-1466 (nad5); 1476-2032 (trnG)
indel coding positions: 512-515 (gpd); 1468-1474 (nad5); 2034-20...