Effects on soil fungal patterns distribution of conservative agricultural practices in a Mediterranean area

Aim of the study was to explore fungal diversity and distribution in a farm where conventional monoculture was compared with a rotation system, in a long-term experiment. In addition to this, a tillage/no tillage contrast was also considered. The sampling scheme used, and the setting of some main environmental variables have been formulated in order to obtain a dataset capable of highlighting the effects of the different soil management onto fungal communities. The study was performed using a metagenomic approach. Analysis were carried out using the Illumina MiSeq system targeting the fungal Internal Transcribed Spacer regions.