Data for the course "Population Genomics" at Aarhus University

Datasets, conda environments and Softwares for the course "Population Genomics" of Prof Kasper Munch.

 Data.tar.gz Contains the datasets and executable files for some of the softwares

Course_Env.packed.tar.gz Contains the conda environment used for the course. This needs to be unpacked to adjust all the prefixes. You do this in the command line by

creating the folder Course_Env:  mkdir Course_Env

untar the file: tar -zxf Course_Env.packed.tar.gz -C Course_Env

Activate the environment: conda activate ./Course_Env

Run the unpacking script (it can take quite some time to get it done): conda-unpack

Course_Env.unpacked.tar.gz The same environment as above, but will work only if untarred into the folder /usr/Material - so use the versione above if you are using it in another folder. This file is mostly to execute the course in our own cloud environment.

environment_with_args.yml The file needed to generate the conda environment. Create and activate the environment with the following commands:

conda env create -f environment_with_args.yml -p ./Course_Env

conda activate ./Course_Env

The data is connected to the following repository: https://github.com/hds-sandbox/Popgen_course_aarhus. The original course material from Prof Kasper Munch is at https://github.com/kaspermunch/PopulationGenomicsCourse.

Description

The participants will after the course have detailed knowledge of the methods and applications required to perform a typical population genomic study.

The participants must at the end of the course be able to:

Identify an experimental platform relevant to a population genomic analysis.

Apply commonly used population genomic methods.

Explain the theory behind common population genomic methods.

Reflect on strengths and limitations of population genomic methods.

Interpret and analyze results of population genomic inference.

Formulate population genetics hypotheses based on data

The course introduces key concepts in population genomics from generation of population genetic data sets to the most common population genetic analyses and association studies. The first part of the course focuses on generation of population genetic data sets. The second part introduces the most common population genetic analyses and their theoretical background. Here topics include analysis of demography, population structure, recombination and selection. The last part of the course focus on applications of population genetic data sets for association studies in relation to human health.

Curriculum

The curriculum for each week is listed below. "Coop" refers to a set of lecture notes by Graham Coop that we will use throughout the course.

Course plan

Course intro and overview:

Coop chapters 1, 2, 3, Paper: Genome Diversity Project

Drift and the coalescent:

Coop chapter 4; Paper: Platypus

Exercise: Read mapping and base calling

Recombination:

Lecture: Review: Recombination in eukaryotes, Review: Recombination rate estimation

Exercise: Phasing and recombination rate

Population strucure and incomplete lineage sorting:

Lecture: Coop chapter 6, Review: Incomplete lineage sorting

Exercise: Working with VCF files

Hidden Markov models:

Lecture: Durbin chapter 3, Paper: population structure

Exercise: Inference of population structure and admixture

Ancestral recombination graphs:

Lecture: Paper: Approximating the ARG, Paper: Tree inference

Exercise: ARG dashboard exercises + Inference of trees along sequence

Past population demography:

Lecture: Coop chapter 4, Paper: PSMC, revisit Paper: Tree inference

Exercise: Inferring historical populations

Direct and linked selection:

Lecture: Coop chapters 12, 13, revisit Paper: Tree inference

Admixture:

Lecture: Review: Admixture, Paper: Admixture inference

Exercise: Detecting archaic ancestry in modern humans

Genome-wide association study (GWAS):

Lecture: Coop lecture notes 99-120

Exercise: GWAS quality control

Heritability:

Lecture: Missing heritability and mixed models review ; Coop Lecture notes Sec. 2.2 (p23-36) + Chap. 7 (p119-142)

Exercise: Association testing

Evolution and disease:

Lecture: Genetic architecture review ; Article about "omnigenic" model ; Coop Lecture notes Sec. 11.0.1 (p217-221)

Exercise: Estimating heritability