Triticum aestivum cv. Chinese Spring Genome sequencing and assembly

Assembling complete genomes for large and intricate plant species remains a formidable task, and the hexaploid wheat genome poses a particularly challenging hurdle among essential food crops. To overcome this challenge, we employed a complementary approach that integrates PacBio HiFi and ONT ultra-long (UL) read sequencing, along with crucial datasets such as chromosome conformation capture (Hi-C), Illumina, and Bionano. Our achievement presents a comprehensive telomere-to-telomere (T2T) wheat genome with no gaps and accurately includes all 21 centromeres and 42 telomeres.