Mid-pass whole genome sequencing of 12 HapMap genomes

We selected 12 HapMap samples (NA12877, NA12878, NA12879, NA18954, NA18995, NA19238, NA24143, NA24149, NA24385, NA24631, NA24694, NA24695) to test low-pass sequencing coverage and data quality targeting the mid-pass (1-7x) coverage range. We generated 10 replicates per sample: 4 replicates at 1x using plexWell LPS384 library preparation, targeting 150Gb total bases; 4 replicates at 4x using plexWell LPS384 library preparation, targeting 600Gb total; 2 replicates at 4x using plexWell WGS24 library preparation, targeting 300Gb total. Libraries were sequenced on NovaSeq 6000 instruments, with 2x151bp reads. Library preparation and sequencing were done at Psomagen.