CCR7 expression in B6.WT mice at day 14 after infection with L. major.

(A) The percentage of CD11c+ cells expressing CCR7 was determined by flow cytometry in the footpad, draining lymph node (LN) and spleen. (B) The expression of CCR7 on CD11b+CCR2+ monocytes was determined in each tissue of B6.WT mice. (C) A representative histogram of CCR7 expression on CD11b+CCR2+ monocytes in each tissue is shown. (D) Monocytic populations were defined according to their expression of Ly6C as shown in Figure 2A, and the percentage of cells in each population expressing CCR7 is shown. (E) The percentage of Ly6G+ neutrophils in each tissue expressing CCR7 was analysed by flow cytometry. Experimental group size: n=5 mice.