Linkage of <i>Tg(flk1:RFP)is18</i> transgene integration site to position 24.219 Mb Chromosome 3.

*Microsatellite STS markers Z7419, G39247, Z5197, Z7486. cbx1a STS is a Short Simple Repeat located in the 5th intron of the cbx1a gene. Genotype determined by size of PCR amplification products after gel electrophoresis. L, long; M, middle; S, short allele PCR products. is18 transgenic genotype (+ or −) confirmed by PCR with primers that amplify across the genomic DNA-transgene junction (3) or amplify an internal fragment of the transgene concatemer (4). #Genotype of is18/+ female and WIK male was determined. Segregation of STS markers in 10 is18/+ and 10+/+ siblings was used to determine the haplotype of the chromosome containing the is18 transgene integration. The Tg(RFP)is18 chromosome haplotype in the region surrounding the integration site is Z7419-L, G39247-S, Z5197-L, cbx1a STS-L. $100 is18/+ and 100+/+ siblings from a cross between a Tg(flk1:RFP)is18/+ female and WIK wild type male were genotyped by PCR. 1/100 Tg(flk1:RFP)is18/+ progeny was homozygous Z7419 M/M and heterozygous G39247 L/S. This indicates a single recombination event between markers Z7419 and G39247 and genetic map distance of 1 centimorgan. Linkage of Tg(flk1:RFP)is18 transgene integration site to position 24.219 Mb Chromosome 3.