Genome-wide maps of chromatin state (H3K9me2 or CENP-A/Cnp1) in wt strains carrying the inactivated Centromere 1 or 2 or Cnp1 spreading with ura4 inserted into the right side of the repetitive sequences of Chromosome 1 (designated as otr1R::ura4)

We report the high-throughput profiling of histone modification (H3K9me2) or histone variant CNEP-A/Cnp1 in fission yeast Schizosaccharomyces pombe. By obtaining 1-10 ng immunoprecipitated DNA, we generated genome-wide H3K9me2 or CENP-A/Cnp1 maps of wild type strains carrying the inactivated Centromere 1 or Centromere 2 or Cnp1 spreading in fission yeast with a reporter gene ura4 cassette inserted into the right side of the repetitive sequences of Chromosome 1 . We find that neocemtromeres are formed preferably at pericentromeric regions ubiquitously and asymmetrically in all three centromeres of these indicated strains. Overall design: 3 wild type strains carrying the inactivated Centromere 2; 7 wild type strains carrying the inactivated Centromere 1 and 5 wild type strains carrying the Cnp1 spreading were examined by anti-CENP-A/Cnp1 ChIP-seq. 2 wild type strains carrying the inactivated Centromere 2 and 1 wild type strain carrying the Cnp1 spreading were examined by anti-H3K9me2 ChIP-seq.