Differential gene expression in M. tuberculosis infected-murine bone marrow-derived macrophages upon colchicine treatment

We investigated the molecular mechanisms by which colchicine regulates host defense in macrophages against M. tuberculosis infection. To elucidate this, we performed global gene expression analysis of M. tuberculosis infected-bone marrow-derived macrophages after colchicine treatment. Bone marrow-derived macrophages (BMDMs) were differentiated from 6~7 weeks old female C57BL/6 mice and then divided into 9 groups 1) Naïve BMDMs 2) M. tuberculosis infected BMDMs 3) M. tuberculosis infected BMDMs followed by colchicine treatment for 4 h 4) Naive BMDMs 5) M. tuberculosis infected BMDMs 6) M. tuberculosis infected BMDMs followed by colchicine treatment for 4 h 7) Naïve BMDMs 8) M. tuberculosis infected BMDMs 9) M. tuberculosis infected BMDMs followed by colchicine treatment for 4 h