Single cell RNA sequencing of iPSC-derived lung organoid models

Here, we used single cell RNA sequencing of iPSC17 WT 7B2-derived day 10 lung spheroids, unsorted 3-, 6-, and 10-week lung progenitor organoids (LPOs) and induced bud tip progenitor organoids (iBTOs) sorted from 4- and 10-week LPOs, sequenced 4 weeks post-sort. All cultures were grown in serum-free basal media supplemented with WNT activator CHIR99021, FGF7, and all-trans retinoic acid ('3F media').