NR4A2 and NR4A3 selectively modulate elements of the monocyte response to buffered hypercapnia

In wild type, NR4A2-deficient, and NR4A3-deficient monocytes we used RNA-sequencing to investigate the effect of 4hrs of exposure to buffered hypercapnia (10% CO2) compared to normocapnia (5% CO2) with and without the pro-inflammatory stimulus LPS (2.5ug/ml) for the final 2hrs. Buffered hypercapnia causes transcriptional changes associated with altered metabolic function in both the basal and stimulated states. Overall design: To investigate the effect of NR4A2 ot NR4A3 depletion on the hypercapnic response in monocytes, we exposed NR4A2-deficient and NR4A3-deficient monocytes to 10% CO2 or 5% CO2 for 4 hours in the presence or absence of LPS (2.5ug/ml) for the final 2 hours.