Essential Role of Sirt2 in Type I Interferon Signaling. Mus musculus

We provide evidence for the essential role of Sirt2 in IFN mediated signaling. Our studies demonstrate that SIRT2 controls expression of key interferon stimulated genes (ISGs) that mediate important biological functions, including anti-viral and antineoplastic responses. Overall design: Total RNA was isolated from untreated and IFNβ-treated Sirt2+/+ and Sirt2-/- mouse embryonic fibroblasts (MEFs). Cells were treated for 4hours with or without mouse IFNβ (2500 IU/ml).