Transcriptional profiles of sov mutant ovaries

Stranded, single-end, polyA+, transcriptional profiles were created from ovaries of sterile and fertile sov heteroallelic mutants and Gal4 driven sov RNAi knockdowns. Overall design: 15 ovaries from 4-5 day old post-eclosion females grown in uncrowded conditions were dissected and pooled for each biological replicate for a total of three replicates per genotype. Total RNA was extracted from tissues and polyA RNA was isolated and used to prepare stranded RNAseq libraries. 50 bp single-end sequencing was performed and mapped to Drosophila melanogaster release 6.21 genome.