Supporting Information S1 - Emergent Functional Properties of Neuronal Networks with Controlled Topology

Analysis of the average neurite extension of mCherry-transfected neurons grown in grid patterns. The average neurite length was evaluated by transfecting a limited number of neurons in the network with mCherry. The transfection protocol demonstrates that the imposition of the pattern did not prevent neurites to develop normally as in standard random cultures. Fig. S1 (left panel) shows that the transfection protocol allowed to effectively track neurites of a single neuron up to 800 µm from the soma. The quantification yielded to an average neurite length of 625±81 µm (n = 4). (DOCX)