RNA seq of cardiac fibroblasts primary cell lines after stimulation with WNT3a or WNT5a with or without TGFB

To recognize the influnence of WNT signaling on fibroblasts differentiation first we analyzed transformation of human cardiac fibroblasts caused by TGF-ß signaling. Differential gene expression analysis demonstrated that cardiac fibroblasts 72h after treatment with TGF-ß showed deregulated expression of 313 genes. We also observed that stimulation with WNT3a resulted in deregulation of 124 genes in TGF-ß-treated fibroblasts and in contrast to profound effect of WNT3a on fibroblasts differentiation, treatment with WNT5a upregulated expression of only 2 and downregulated 21 genes in TGF-ß-activated cells. Overall design: Examination of effect WNT3a and WNT5a on cardiac fibroblasts differentiation