Influence of IWS1 TND-interacting motifs (TIMs) on chromatin localization patterns [ChIP-seq]

To reveal the influence of TND-interacting motifs (TIMs), which bind TFIIS N-terminal domains (TNDs), we prepared KELLY cells expressing either wild-type IWS1-FLAG or IWS1-FLAG containing disruptive mutations in the unstructured TIM3 region. Following lentiviral transduction of either WT or M3 IWS1-FLAG, we performed ChIP using anti-FLAG antibodies from these samples and performed chromatin occupancy profiling using ChIP-seq. Analysis of locations reveal that both WT and M3 samples are highly enriched in at transcribed gene bodies. Anti-FLAG ChIP-seq in KELLY cells expressing wild-type (WT) or mutant (M3) IWS1-FLAG via lentiviral transduction.