Microbiome characterization using transfer RNA sequencing

Here we report a direct tRNA sequencing protocol and software to simultaneously examine the composition and biological activity of naturally occurring microbial communities. Our analysis of mouse gut microbiome with tRNA-seq and 16S ribosomal RNA gene amplicons revealed comparable microbial community structures, and additional physiological insights into the microbiome through tRNA abundance and modifications. Overall design: Comprehensive analysis of high throughput sequencing data to determine microbial community structures in mouse gut microbiome (tRNA profiling dataset).