QuantitaQuantitative proteomic analysis of β-cells response to thapsigargin and rotenone uncovers key pathways of rat β-cell dysfunction tive proteomic analysis of β-cells response to thapsigargin and rotenone uncovers key pathways of rat β-cell dysfunction

We designed a TMT peptide labeling-based quantitative proteomics strategy to achieve a high-accuracy analysis of proteomic changes in response to ER stress and mitochondria stress on INS-1 cells under three different culture conditions: control (Cont), rotenone (Rot), and thapsigargin (Tpg), each with five biological replicates. The TMT data was validated using label-free quantification method.