RNA-seq of a foxtail millet mutant siago1b

Setaria italica mutant siago1b and wild-type (WT) Yugu1 plants were grown in a growth chamber for 3 weeks. The aboveground parts of siago1b and WT plants were used to extract total RNA for transcriptome sequencing. RNA quality and purity were examined using an Agilent Bioanalyzer 2100. The cDNA library was constructed following the Illumina sequencing manual. The cDNA libraries of mutant siago1b and the WT were sequenced on an Illumina HiSeq 2000 Genome Analyzer (Illumina, San Diego, CA, USA) with three independent replicates.