Gliomedin drives gastric cancer cell proliferation and migration, correlating with a poor prognosis

Total RNAs were extracted from stable AGS/GLDN-OV and control cells, which were used for cDNA library construction and paired-end reads sequencing. Analysis and data mining were performed using the Dr. Tom Multi-omics Data mining system, as described previously. Briefly, after the raw data obtained by RNA-sequencing was subjected to quality control, the clean reads obtained by filtering with SOAPnuke were aligned to the reference genome sequence using HISAT, and Bowtie2 was used to compare the clean reads with the reference genes. Finally, quantitative and differential gene expression analyses were performed after the second quality control step. Three replicates were prepared, sequenced, and analyzed separately.