Specific IgE values of commercial and in-house-made ImmunoCAPs including HRP-mediated CCD inhibition for selected patients.
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CCD inhibition was done by incubating the respective patient sera over night at 4°C with HRP (10 mg/ml final concentration). Arabidopsis was included as control.‡When conducted with a final inhibitor concentration of 33.3 mg/ml HRP, the sIgE value decreased to 0.22 kU/l (below the 0.35 kU/l threshold, compare Figure S5B). (BW: bee/wasp hymenoptera venom-allergic; cgl1: complex glycan-less GNTI-null mutant; f25: commercial tomato ImmunoCAP; f35: commercial potato ImmunoCAP; GTI: GNTI-silenced; PT: potato/tomato-allergic; wt: wild-type).
创建时间：
2015-12-02
