LDDN-0003499 treatment attenuated total protein phosphotyrosine levels in BV2 cells.

Microglial BV2 cells were treated with vehicle (DMSO), 0.5nM, 5nM, 50nM, 0.5 μM, 5μM, and 50 μM (A) LDDN-0003499, (B)LDDN-0125694, (C) LDDN-0075935, and (D) LDDN-127164 for 1h. Cell lysates were resolved via SDS-PAGE and western blotted using anti-phosphotyrosine (4G10) antibody with α-tubulin as the loading control. A representative western blot is shown. Optical densities from three independent experiments were graphed and averaged ± SD (*p<0.05 vs. vehicle).