Single cell RNA sequencing of peripheral retina in control and FGF signaling mutant mouse embryonic tissue

The goal of the study is to identify changes in gene expression and cell distribution in the ciliary margin upon loss of FGF signaing. Neural retinal cells from embryonic day E14 were isolated from control and mutant eyes. Dissociated cells were sorted based on GFP and TdTomato reporter expression in the control and mutant retina respectively. Flow-sorted single cells were processed by the core facility at Columbia University on a 10x platform. Overall design: 3 control and 3 mutant embryos were pooled and sorted based on reporter expression for single cell sequencing