RT-PCR of microRNAs derived from rats: Ischaemia, Sevoflurane preconditioning, Ischaemic preconditioning, Sham model

We compared sevoflurane preconditioning and ischaemic preconditioning effects against renal ischaemia reperfusion injury via microRNAs of the kidney that promote cell-survival pathways in rats. In our protocol, male Wistar rats were divided into four groups (Ischaemia, Sevoflurane preconditioning, Ischaemic preconditioning and Sham models; n=7 each) and all underwent right nephrectomy. Ischaemia model rats underwent 45-minute clamping of left kidney, followed by 4-hour reperfusion. Sevoflurane preconditioning involved exposure to 1 MAC sevoflurane for 15 minutes. Ischaemic preconditioning included 3 cycles of 2-minute clamping and 5-minute reperfusion. Renal biopsy samples were assessed postoperatively to comprehensively analyse renal mircoRNAs using RT-PCR and the predicted cell-survival pathways. Sevoflurane preconditioning and ischaemic preconditiong ameliorated ischaemia reperfusion injury, indicated by serum creatinine equally. The result of RT-PCR indicated that sevoflurane and ischaemic preconditioning affected different kinds of microRNAs related to the cell-survival pathways respectively.