Injection of alpha-synuclein preformed fibrils into mouse M2 cortex impairs corticostriatal glutamate transmission compared to monomer injected mice

Ex-vivo slice recordings from mice co-injected with AAV8-hSyn-hChR2-tdTOMATO and either monomeric a-synuclein or synuclein preformed fibrils (PFFs) in the M2 cortex were assessed 6w post-injection via optogenetic stimulation of cortical afferents. This dataset includes data from whole--cell patch clamp recordings including spontaneous and evoked EPSCs, paired-pulse ratios, asynchronous synaptic events and intrinsic excitability. The data confirms there is a physiological difference between M2-PFF and M2-monomer injected animals as shown by a decrease in evoked excitatory current amplitude in the PFF-injected mice. The asynchronous release experiments showed fewer events in PFF-injected mice suggesting a reduction in active release sites.