Mechanical testing of cyanobacterial colonies

Experimental data and image analysis scripts associated with the manuscriptSinzato et al, "On Linear and Non-Linear Mechanics of Cyanobacterial Colonies", in submissionThree experimental datasets and associated data analysis scripts are deposited:Bulk shear rheology:Large amplitude oscillatory shear (Rheology/LAOS/): Csv files with rheometer readoutConfocal microscopy images (Rheology/Microscopy): tif files before and after shear rheologyScript for analysis of microscopy images (Rheology/Microscopy/confocal_analysis.ipynb): jupyter notebookMicropipette force sensing:Raw images of micropipette force calibration (Micropipette force sensing/Raw pipette calibration/): tif image stacksRaw images of micropipette pulling of individual colonies of Microcystis (Micropipette force sensing/Raw force measurement/): tif image stacksProcessed data (Micropipette force sensing\Processed data/Results): csv files with detected trajectories, measured forces and morphological analysisImages for morphological analysis (Micropipette force sensing/Processed data/Colony Raw Images): tif filesScript for calibration of micropipettes (Micropipette force sensing/Processed data/Micropipette Calibration/Droplet force calibration.ipynb and Relative force calibration.ipynb): jupyter notebook for extraction of micropipette bending stiffnessScripts for analysis of force measuresements (Micropipette force sensing/Processed data/Force_detection.ipynb): jupyter notebook for tracking micropipettes, calculate forces, measure morphological features, compare and plot resultsStaining of EPS in colony:Raw and processed confocal images of stained colonies (Staining with DTAF): tif files