MICrONS Two Photon Functional Imaging

The light microscopic images were acquired from a cubic millimeter volume that spanned portions of primary visual cortex and three higher visual cortical areas. The volume was imaged in vivo by two-photon random access mesoscope (2P-RAM) from postnatal days P75 to P81 in a male mouse expressing a genetically encoded calcium indicator in excitatory cells, while the mouse viewed natural movies and parametric stimuli. The calcium imaging data includes the single-cell responses of an estimated 75,000 pyramidal cells imaged over a volume of approximately 1200 x 1100 x 500 μm3 (anteroposterior x mediolateral x radial depth). The center of the volume was placed at the junction of primary visual cortex (VISp) and three higher visual areas, lateromedial area (VISlm), rostrolateral area (VISrl) and anterolateral area (VISal). During imaging, the animal was head-restrained, and the stimulus was presented to the left visual field. Treadmill rotation (single axis) and video of the animal's left eye were captured throughout the scan, yielding the locomotion velocity, eye movements, and pupil diameter data included here.

The functional data were co-registered with electron microscopy (EM) data. The structural identifiers of the matched cells are added as plane segmentation columns extracted from the CAVE database. To access the latest revision see the notebook that is linked to this dandiset. The structural ids might not be present for all plane segmentations.