Differential N6-methyladenosine(m6A) modification in hypertrophic scar v.s. normal skin

N6-methyladenosine (m6A) is the most prevalent internal modification in eukaryotes. Here, m6A-mRNA and lncRNA transcriptomes were profiled for HS and full-thickness normal skin (NS) tissues using human m6A-mRNA and lncRNA epitranscriptomic microarray. These data exhibited 5838 differentially m6A methylated transcripts (4706 mRNAs, 1132 lncRNAs) between HS and NS tissues. Of note, most of them (about 94%) were hypermethylated. And 3197 differentially expressed mRNAs (1466 upregulated and 1731 downregulated) were obtained by microarray. Conjoint analysis of differentially methylated and differentially expressed transcripts screened 815 transcripts (675 hyper-up, 140 hypo-down). Among them, IFNGR1 (ENST00000367739) and IL37 (ENST00000349806) are two of the most significant methylated transcripts in hyper-up and hypo-down groups. This study proposed a transcriptional regulatory network of m6A modifications on mRNA and lncRNA in human hypertrophic scar, which may bring a new clue into the development, pathogenesis, and treatment of abnormal scars. 4 samples(2 hypertrophic scars,2 normal skin); Please note that experiments were carried out technically as dual channel (eg, Cy3 and Cy5-labeled samples hybridized to the same array) but processed as single channel. Thus each raw data file contains raw data for both IP and supernatant sample, and is linked to the corresponding IP sample records.