Cell lysis conditions applied to obtain maximum DNA for the next-generation sequencing of the microbial community in the various segments of the GI tract.
收藏Figshare2015-12-02 更新2026-04-29 收录
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https://figshare.com/articles/dataset/_Cell_lysis_conditions_applied_to_obtain_maximum_DNA_for_the_next_generation_sequencing_of_the_microbial_community_in_the_various_segments_of_the_GI_tract_/1258236
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1100 mg/ml (Applycehm). 2Cetyltrimethylammonium bromide (w/v). 31 M Tris-HCl 100 ml, 500 mM EDTA 50 ml, 5 M NaCl 300 ml, 10% CTAB, 20% SDS, pH = 829. 4ASL buffer from Qiagen QIAamp DNA Stool miniprep kit (Qiagen, 51504). 5From Zymo Research Fecal DNA kit (Zymo Research, D6010).Cell lysis conditions applied to obtain maximum DNA for the next-generation sequencing of the microbial community in the various segments of the GI tract.
创建时间：
2015-12-02
