Data underlying the publication: Noise amplification and ill-convergence of Richardson-Lucy deconvolution

The dataset pertains to a study on noise amplification and sensitivity for Richardson-Lucy deconvolution of widefield fluorescence microscopy image data. The image data pertains to the MitoTracker red channel (emission wavelength 599 nm) and Alexa Fluor 488 Phalloidin channel (emission wavelength 512 nm), labeling mitochondria and actin, respectively, of a sample of Bovine Pulmonary Arterial Endothelial cells (BPAE-cells, ThermoFisher FluoCells \#1), imaged with a Nikon Ti-E microscope body with a 40X/NA0.95 objective lens with 1.5X magnification adapter, equipped with an sCMOS camera (Teledyne Kinetix, 3200 3200 pixels, pixel size 6.45 micron. In addition, a set of dark images was recorded for the assessment of offset and readout noise, and a through-focus bead measurement for PSF/OTF assessment.

本数据集关联一项针对宽场荧光显微镜（widefield fluorescence microscopy）图像数据的理查德森-露西去卷积（Richardson-Lucy deconvolution）噪声放大特性与灵敏度分析研究。该图像数据对应牛肺动脉内皮细胞（BPAE细胞，赛默飞世尔FluoCells #1）样本的两个成像通道：分别用于标记线粒体的MitoTracker红色通道（发射波长599 nm），以及用于标记肌动蛋白的Alexa Fluor 488鬼笔环肽通道（发射波长512 nm）。成像采用尼康Ti-E显微镜主机，搭配40×/NA0.95物镜及1.5×放大适配器，搭载Teledyne Kinetix型号sCMOS相机，该相机分辨率为3200×3200像素，像素尺寸6.45微米。此外，本研究还采集了一组暗场图像用于偏移量与读出噪声评估，并通过轴向聚焦微球测量完成点扩散函数/光学传递函数（PSF/OTF）的表征。