Mutant Harmonia axyridis raw sequencing data of CRISPR/Cas9 induced mutations in the genes laccase2 and scarlet

Harmonia axyridis (Pallas), commonly known as the Asian lady beetle, is a
native insect species of Asia that has been intentionally introduced to
various regions for biocontrol purposes. However, its widespread presence
beyond its original release sites suggests a high degree of invasiveness.
In this study, we utilized the CRISPR-Cas9 approach to achieve precise
genome editing in H. axyridis. Specifically, we targeted two genes in H.
axyridis, laccase2 and scarlet, knockdown of which orthologs in other
insects showed visible phenotypic changes. The knockout laccase2 resulted
in an early-detectable phenotype but also in lethality. However, we
successfully established a viable and genetically stable mutant colony by
disrupting the scarlet gene, resulting in beetles with white eyes. Our
findings contribute to the expanding knowledge of genetic manipulation in
H. axyridis and provide insights into its potential for future research
and practical applications for biocontrol and invasive species management.