Number of endocytic vesicles labelled with FM1-43 and pHrodo Green dyes.

Comparing control (22 cells at pH 7.25 and 27 cells at pH 5.5) to exposure to 5 µM Bleb (33 cells at pH 7.25 and 26 cells at pH 5.5) and to 4 µM Cyto (21 cells at pH 7.25 and pH 5.5). The pairs of data sets were compared using a two-tailed U-Mann-Whitney rank-sum test, and the result is indicated, between brackets, next to the variation of the mean. Numbers are presented as means ± SEM. Bleb, Blebbistatin; Cyto, Cytochalasin D.