VEGF-Induced Transcriptomic Alterations in A549 Cells

To investigate the mechanisms of cellular transformation, we performed transcriptome sequencing (RNA-seq) on VEGF-treated A549 non-small cell lung cancer (NSCLC) cells, with a focus on profiling changes in key target genes. Overall design: A549 cells were divided into two groups: a vehicle control group and a VEGF treatment group. Each group consisted of two biologically independent replicates. Following the treatment, total RNA was isolated from all samples. The integrity and quantity of the RNA were verified. Subsequently, RNA-seq libraries were constructed from the high-quality RNA and sequenced on an Illumina platform to generate transcriptome profiles for subsequent analysis.