Application of virus-induced gene silencing in <i>Pogostemon cablin</i> advances our understanding of patchoulol and sesquiterpene metabolism

In plant species, virus-induced gene silencing (VIGS) effectively enables the transient knock down of target genes for functional analysis. To overcome the bottleneck preventing the functional genomics and biological engineering of <i>Pogostemon cablin</i> (Blanco) Benth. an important medicinal and industrial plant with high-value natural products, the VIGS system was established to enable the fast and efficient functional characterisation of candidate genes. In our study, the effectiveness of phytoene desaturase (PDS) gene silencing by VIGS was monitored by the visual indications in P. cablin and the activation effect spanned several months. VIGS of the PatPTS gene, encoding patchoulol synthase, caused a decline in expression abundance, resulting in a significant decreasing accumulation of patchouli alcohol and other sesquiterpenes. Cosilencing of PatPDS and PatPTS in <i>P. cablin</i> also suppressed the biosynthesis of patchouli alcohol. Negative feedback of the MVA and MEP pathway genes was demonstrated in PatPTS-silenced plants.

在植物物种中，病毒诱导基因沉默（virus-induced gene silencing, VIGS）可有效实现靶基因的瞬时敲降，用于功能分析。为破解广藿香（Pogostemon cablin (Blanco) Benth.）功能基因组学与生物工程研究的瓶颈——该物种是一类富含高价值天然产物的重要药用与工业植物，本研究建立了VIGS体系，以实现候选基因的快速高效功能鉴定。
本研究通过广藿香植株的视觉表型变化，监测了八氢番茄红素脱氢酶（phytoene desaturase, PDS）基因经VIGS介导的沉默效果，该沉默效应可维持数月之久。
对编码广藿香醇合酶的PatPTS基因进行VIGS沉默后，其转录表达丰度显著降低，导致广藿香醇及其他倍半萜类物质的积累量明显下降。
在广藿香中共沉默PatPDS与PatPTS基因，同样会抑制广藿香醇的生物合成。
对PatPTS沉默植株的分析表明，甲羟戊酸（MVA）与甲基赤藓醇磷酸（MEP）途径的基因存在负反馈调控现象。

搜集汇总

背景与挑战

背景概述

该数据集聚焦于应用病毒诱导基因沉默（VIGS）技术在广藿香（Pogostemon cablin）中研究广藿香醇和倍半萜代谢，通过沉默关键基因如广藿香醇合酶（PatPTS），揭示了其表达降低导致广藿香醇积累减少及代谢途径的负反馈机制。数据集包含实验图表和表格，发布于2022年，支持功能基因组学和植物生物学研究，适用于医学、遗传学及植物科学领域。