Structure and functional mapping of the KRAB-KAP1 repressor complex [KAP1_ChIP-seq]. Structure and functional mapping of the KRAB-KAP1 repressor complex [KAP1_ChIP-seq]

We used ChIP-seq to assess how abolition of KRAB binding by KAP1 (through structure-guided mutations in the KAP1-KRAB binding interface) changed the distribution of KAP1 on chromatin genome-wide. Overall design: Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for KAP1 in three different conditions [KAP1-KO, WTcomp (KAP1-KO cells complemented with a wild type KAP1), and CCmutComp (KO cells complemented with a KAP1-coiled coil mutant)]. Experiments were performed in HEK293T cells (n=2 for each condition).