Ferroxidase activity, HepG2-, C6- and CG4-conditioned media, but not iron chelators stimulate hBMVEC iron efflux.

Media in bold was supplemented with components listed or were conditioned for 24 h with the cell type as indicated; “Control” media was RPMI1640. Neural basal and M41 media are defined in “Materials and Methods”. Cells were loaded with 59Fe-Transferrin (59TBI) instead of 59Fe-citrate in one experiment as indicated. The FD1 peptide sequence: was HRERMSQVMREWEEAERQAKNL. CG4 is a bipotential oligodendroglial precursor cell line. A series of paired t-tests were used to analyze the data at each time point for each condition in comparison to Control Media.
*P<0.05,
**P<0.01,
***P<0.001,
****P<0.0001. Data are represented as means ± S.D. (n = 4–8; experimental replicates).