Effects of dopamine on HIV latently-infected U1 macrophage cell line

U1 monocytic cell line was cloned from U937 cells that survived an infection with Human Immunodeficiency Virus HIV-1 and bear integration sites, serving as one of the most studied HIV latent models. Under the hypothesis that the hyperdopaminergic environment of the brain of substance users affects HIV-infected cells and latency, we tested the effects of Dopamine and two dopamine receptors that were identified as able to signal changes ininnate immune cells, DRD1 and DRD4. U1 cells were exposed for 24 hrs to Dopamine 1uM, SKF 38393 (1uM DRD1 selective agonist), and PD 168077 (1uM DRD4 selective agonist). The concentrations were pre-determined in dose-response experiments, and