Sensitivity and PCR efficiency of SensiScreen<sup>®</sup> simplex and multiplex assays determined by serial dilutions of mutated DNA in a wild type background.
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50 ng and/or approximately 16,000 copies of DNA was added to each real-time PCR mixture. The threshold was set at 10% of the average fluorescence signal of the reference assay at cycle 45.
创建时间：
2017-06-22
