Estimates of virus accumulation, virus effects on vegetative and reproductive growth, and virulence in horizontally inoculated Cen-1 plants derived from the fifth vertical transmission passage.

1Accumulation of virus RNA (µg/g fresh weight) estimated for 1∶1 mix of inoculated and systemically infected leaves. 2Effect of CMV infection on rosette weight (RW) and inflorescence weight (IW) estimated as Traiti/Traitm, where i and m denote infected and mock-inoculated plants, respectively. 3Virulence estimated as one minus the ratio of seed weight in infected vs. mock-inoculated plants: 1−(SWi/SWm). Values are mean±standard error of the 5 lineages in evolved viruses and 10 replicates in non-evolved strains. Large arrows indicate values significantly higher (↑) or lower (↓) than the equivalent value in Table 2 (P≤0.05), and small arrows indicate non-significant trends (P<0.10).