Structural stability of the wild type EcPA at pH 7.5 and 10.0.
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Root mean square deviation (RMSD) for each enzyme variant has been averaged over three independent MD runs. The mean and standard deviation are shown in angstroms. Nres – number of amino acid residues in a structural domain. ΔRMSD = RMSDpH10 – RMSDpH7.5 and f = f(RMSDpH7.5 ≥ RMSDpH10) were calculated as explained in Methods, section 2. Lower f values indicate a significant destabilizing effect of the alkaline pH compared to neutral conditions while ΔRMSD estimates the degree of destabilization.
创建时间：
2015-12-02
