RNA-sequencing of dura mater or brain ECs isolated from 8-week-old mice

To further elucidate fundamental molecular differences between dura mater and brain ECs, we performed RNA-sequencing of primary dura mater or brain ECs isolated from adult mice Dura mater or ECs were isolated by FACS from 8-week-old mice and mRNA profiles were generated by deeping sequencing, in duplicate, using Illumina NextSeq 500.