Supplementary material.docx

Experimental.Plant materials and phytopharmaceutical productChemicals and apparatusPreparation of Standards and samplesInstrumentation and Chromatographic conditionsFiguresOptimisation of chromatographic conditions<b>S1:</b>Effect of organic solvent type in the mobile phase on separation <b>S2:</b>Effect of initial and final concentration of ACN in the mobile phase on separation <b>S3:</b>Effect of gradient time on separation <b>S4:</b>Effect of column temperature on separation <b>S5:</b>Effect of flow rate on corrected peak area <b>S6:</b>Peak characterization in the crude extracts Tables<b>S1:</b>Optimum chromatographic conditions Determination of analytes in real samples <b>S2:</b>Concentration of analytes in the real samples <br>