Mammary intraepithelial lymphocytes promote lactogenesis and offspring fitness

To test the role of intraepithelial lymphocytes in mammay grand epithelial function during pregnancy we performed single cell RNA sequencing of epithelial and myoepithelial cells from the mammary gland in WT mice, Raggc-KO mice, Raggc-KO mice adaptively transferred with IEL precursors. Overall design: For mammary epithelial and myoepithelial scRNA-seq, thymic IELp were adoptively transferred (+IELp) or not (-IELp) into pregnant Rag2-/-?c-/- /- at day 10.5 of gestation. Mammary epithelial cells and myoepithelial cells (CD45-CD31-EPCAM+) from Rag2-/-?c-/- mice with or without IELp were sorted 8 days post-transfer (G18.5) from samples labeled with TotalSeqC hashtags antibodies (Biolegend) with a Sony MA900 sorter. Sorted cells were pooled together and 45,000 cells were loaded to a Chromium Single Cell Controller (10X Genomics) to encapsulate the cells into droplets (1 lanes).