Preventive administration of soluble Robo4 (sR4) reduces lesion severity in HSV-1 infected mice.

WT mice were infected with HSV-1 RE and treated with sR4 or vehicle (PBS) (A) The sR4 treatment was given to HSV-1 infected mice as shown (B) Dose dependent inhibition of angiogenesis scores after sR4 treatment. Data represents means ± SEM from three different experiments (n = 10) (C) sR4 treatment regimen resulted in SK and angiogenesis scores reduction. Data are representative of three independent experiments and show mean values ± SEM (n = 15 mice/group). ***p ≤ 0.001, **p ≤ 0.01, *p ≤ 0.05. Statistical levels of significance were analyzed by t test. (D) Representative eye photos show decreased SK lesion and angiogenesis severity in sR4 treated mice compared to control mice (E) Eyes were processed for cryo-sections on day 15 pi. Hematoxilyn and eosin staining was carried out on 6 μm sections, and pictures were taken at 40x magnification. The sections show decreased cellular infiltration in mice treated with sR4 compared to control mice.