Expression data from human kidney organoids treated with the Hedgehog agonist SAG

The kidney, similar to non-renal tissue, is adversely affected by increased Hedgehog signaling. We treated human kidney organoids with the Hedgehog activator SAG in order to study the effects of constitutive active Hedghog signaling on human kidney development. We used microarrays to identify gene expression changes in human kidney tissue following Hedgehog activation. Induced pluripotent stem cells were differentiated for 7 days to intermediate mesoderm cells. At the stage of cellular aggregation, kidney organoids were treated with either DMSO (control) or 120 nM SAG. RNA was collected from kidney organoid tissue after 18 days of treatment (at day 25).