Endpoint phenotyping files

The folder contains .ods files of endpoint phenotyping data.Biologically, the data were generated by BY × CEN.PK biparental (back)crossing of two Saccharomyces cerevisiae yeast strains. Phenotype, i.e. NaCl salt tolerance of the generated haploid segregants was measured by endpoint phenotyping. The phenotyping procedure included monitoring growth on solid media plates, supplemented with NaCl, of which images were acquired with scanner. End-point colony sizes were extracted by HT Colony Grid Analyzer from the acquired images.The files contain the phenotype distribution data. The most salt tolerant haploid segregant of each generation was selected based on its tolerance against NaCl and crossed back with one of its parental yeast strains (BY or CEN.PK) for seven generations of crossing. In the F1 generation 2,156 segregants were analysed, whereas in the subsequent generations the analysed number was 1,232. The growth of each segrergant was assessed on NaCl concentrations from 0.25 M to 2.50 M in increment of 0.25 M and was monitored daily for four consecutive days.In each file, there are spreadsheets corresponding to the NaCl concentration (0.25 M, 0.50 M, 0.75 M, 1.00 M, 1.25 M, 1.50 M, 1.75 M, 2.00 M, 2.25 M, 2.50 M) and time of growth when the image was acquired (24 h, 48 h, 72 h, 96 h). Flags correspond to collonies at the border of the agar plate or contamination.