<i>ACVR2</i> expression and mechanisms for loss of expression in colon cancer cell lines.

Using colon cancers cells with different microsatellite instability backgrounds, we confirm wild type A8 exon 10 polyadenine tract in all MSS colon cancer cell lines. As previously published, the MSI colon cancer cell line HTC116 harbors biallelic A8 to A7 frameshifts, leading to loss of full length ACVR2 protein [12]. Two MSI cell lines, SW48 and RKO, harbor mono-allelic mutations with no effect on ACVR2 gene expression. The MSS colon cancer cell line HT29 revealed full ACVR2 promoter hypermethylation, akin to ACVR2 loss in primary human tumors, and was associated with loss of ACVR2 mRNA and protein.